Can you prepare 1 liter of a 0.5-molar glucose solution? This question is commonly encountered in various scientific and medical fields, where precise concentration of solutions is crucial for experiments and treatments. In this article, we will guide you through the process of preparing a 0.5-molar glucose solution, ensuring accuracy and safety.
Firstly, it is essential to understand the concept of molarity. Molarity is a measure of the concentration of a solution, defined as the number of moles of solute per liter of solution. In this case, we aim to prepare a 0.5-molar glucose solution, which means there will be 0.5 moles of glucose in every liter of the solution.
Before starting the preparation, gather the following materials:
- Glucose powder (anhydrous glucose or glucose monohydrate)
- Distilled water
- 1-liter volumetric flask
- Electronic balance
- Pipette
- Beaker
- Measuring cylinder
- Filter paper
Next, follow these steps to prepare the 0.5-molar glucose solution:
- Calculate the amount of glucose required. The molar mass of glucose is approximately 180.16 g/mol. To prepare a 0.5-molar solution, you will need 0.5 moles of glucose, which is equal to 90.08 g.
- Using an electronic balance, weigh out 90.08 g of glucose powder. Ensure that the balance is calibrated and zeroed before weighing.
- Transfer the glucose powder to a beaker, and add a small amount of distilled water to dissolve the glucose. Stir the mixture until the glucose is completely dissolved.
- Filter the solution using filter paper to remove any undissolved particles. This step is crucial to ensure the accuracy of the concentration.
- Pour the filtered glucose solution into a 1-liter volumetric flask. Rinse the beaker with distilled water and add the rinse water to the flask to ensure that all the glucose is transferred.
- Add distilled water to the flask until the bottom of the meniscus is at the calibration mark. This mark indicates the 1-liter volume of the flask.
- Cap the flask and invert it several times to mix the solution thoroughly.
Now, you have successfully prepared 1 liter of a 0.5-molar glucose solution. This solution can be used for various applications, such as cell culture, medical treatments, or research experiments. Always remember to handle chemicals and solutions with care, and dispose of them properly to avoid any environmental or health hazards.
